Method 3: Disable Desktop Overlay When Using Media Keys Feature
Note: This is applicable for people who are experiencing this issue with the Spotify app.
There is an option named Desktop Overlay when using media keys in the Spotify application. Disabling this option has resolved the issue for the majority of Spotify users. Follow the steps given below to change these settings.
Open the Spotify app
Click Options (or Settings)
Uncheck the option Desktop overlay when using media keys
This should solve the issue and your media keys should start working on the Spotify app now.
I found this on Appuals website in the post called "How to Fix Media Keys Not Working on Windows 10" (I can't put the URL because it doesn't if you try to access from here) and for me worked after I disabled google play from chrome extension. Check the Spotify one.
